AdiLight-1 Activation

AdiStem Ltd. has been researching the effect of different monochromatic light intensities and frequencies in the colored spectrum on various human and animal cell populations such as mesenchyme stem cells and white blood cells.

Low-level light photoactivation or photomodulation can be utilized for significant benefit in the stimulation of proliferation, differentiation, and inhibition/induction release of growth factors/cytokines of cells from any living organism.

The wavelength or bandwidth of wavelengths is one of the critical factors in selective photomodulation. Pulsed or continuous exposure, duration and frequency of pulses (and dark 'off' period) and energy are also factors as well as the presence, absence or deficiency of any or all cofactors, enzymes, catalysts, or other building blocks of the process being photomodulated.

Different parameters with the same wavelength may have very diverse and even opposite effects. When different parameters of photomodulation are performed simultaneously, different effects may be produced. When different parameters are used serially or sequentially, the effects are also different.

The selection of wavelength photomodulation is critical as is the bandwidth selected as there may be a very narrow bandwidth for some applications -- in essence these are biologically active spectral intervals. Generally the photomodulation will target flavins, cytochromes, iron-sulfur complexes, quinines, heme, enzymes, and other transition metal ligand bond structures though not limited to these.

These act much like chlorophyll and other pigments in photosynthesis as 'antennae' for photo acceptor molecules. These photo acceptor sites receive photons from electromagnetic sources such as those described in this application, but also including radio frequency, microwaves, electrical stimulation, magnetic fields, and they may also be affected by the state of polarization of light. Combinations of electromagnetic radiation sources may also be used.

The photon energy being received by the photo acceptor molecules from even low intensity light therapy (LILT) is sufficient to affect the chemical bonds thus 'energizing' the photo acceptor molecules which in turn transfer and may also amplify this energy signal. An 'electron shuttle' transports this to ultimately produce ATP (or inhibit) the mitochondria thus energizing the cell (for proliferation or secretory activities for example). This can be broad or very specific in the cellular response produced.

AdiStem has ongoing international research projects looking at the effects of different frequencies of monochromatic lights on various cells including mesenchyme stem cells and white blood cells. It has now found five frequencies (three are present in AdiLight-1) that can activate stem cells, in vitro, and two frequencies that inhibit them. AdiStem has also found similar frequencies to modulate pro-inflammatory and anti-inflammatory cytokine release from peripheral blood white blood cells. AdiStem is also exploring the direct effect of different low level frequencies of light on endogenous cells (in vivo).

AdiLight-1

AdiLight-1 is the first LED device made available commercially by AdiStem for use in activating mesenchyme stem cells and modulating cytokine release by white blood cells.

Mesenchyme Stem Cells

When adipose-derived mesenchyme stem cells are taken out of a subject most of the cells are in a dormant state. In the body, stem cells and progenitor cells need to be activated by a physiological repair mechanism cascade, for example release of growth factor and chemokines by platelets. When the adipose-derived stem cells are photoactivated for 20 minutes with the AdiLight-1 device they show increased proliferation (see Figure 1), increased production of integrins, vascular endothelial growth factor, thymosin beta 4 and interleukin 1 receptor antagonist. Hence, the AdiLight-1 is of value in providing consistent clinical results, especially amongst age differences.

Without AdiLight-1 Exposure	With AdiLight-1 Exposure
Figure 1. Adipose-derived stem cells colonies without (left) and with (right) 20 minutes of AdiLight-1 exposure before plating.

Two Kits are available for the use of AdiLight-1 in the extraction of adipose-derived stem cells. See AdiLight-1 ADSC Kits.

Peripheral Blood White Blood Cells

For many years internal medicine specialists in Eastern Europe and Korea have been using the photoactivation of blood, in vitro and in vivo, with various frequencies of light for immunomodulation in patients. When peripheral blood white blood cells (WBC) are photoactivated under AdiLight-1 for 10 minutes, an inhibition of pro-inflammatory cytokines (IL1, IL2, IL6 and TNFalpha) and induction of anti-inflammatory cytokines (IL1Ra and IL10) and beta endorphins are observed (see Figure 2).

Figure 2. Elisa assay for plasma interleukin 1 receptor antagonist following AdiLight-1 10-minute exposure on four patients' peripheral WBCs before (left) and after (right).

Because of this property we have found AdiLight-1 to be a beneficial add-on to commonly used platelet rich plasma procedures in orthopedic and sports medicine procedures. One of the largest clinical drawbacks of the use of PRP in musculoskeletal healing is the aggravation of pain observed in the injected area post injection. Working with a group of Australian sports medicine specialists, we have deduced that a 10-minute exposure of WBC and platelets to AdiLight-1 prior to injection eliminates the aggravation of pain and potentiates the accelerated healing of PRP. It combines the benefit of autologous conditioned serum (ACS) with PRP in a simple 10-minute exercise.

Although AdiLight-1 is an add-on feature for use with PRP made with any commercially available Kit, we have one commercially viable Kit for deriving PRP/ACS available for ordering.

How to Order

AdiLight-1 is commercially available for use by licensed medical practitioners. Please contact us for pricing and ordering instructions. This patented laboratory device is manufactured in Australia and is made to order.

See Adipose-Derived Stem Cell Activation Science & Technology »

References

Mvula B., Moore TJ & Abrahamse H. Effect of low-level laser irradiation and epidermal growth factor on adult human adipose-derived stem cells. Lasers Med Sci, 2009.

Mvula B, Mathope T, Moore T, Abrahamse H. The effect of low level laser irradiation on adult human adipose-derived stem cells. Lasers Med Sci. 2008

Tuby H., Maltz L and Oron U. Implantation of Low-Level Laser Irradiated Mesenchymal Stem Cells into the Infarcted Rat Heart Is Associated with Reduction In Infarct Size and Enhanced Angiogenesis. Photomedicine and Laser Surgery 27(2): 227-234, 2008.

M. Reale, C. Orso, M.L. Castellani, R.C. Barbacane, F.C. Placido, E. Porreca, C. Di Febbo, I. Cataldo, D. Vacalis, G. Anogianakis, A. Trakatellis and P. Conti. Infra-red laser irradiation enhances interleukin-1 receptor antagonist, increases 3H-thymidine incorporation and the release of [3H]arachidonic acid in human monocytes. Molecular and Cellular Biochemistry 169: 51-59, 1997.

Zhevago N and Samoilova K. Pro- and Anti-inflammatory Cytokine Content in Human Peripheral Blood after Its Transcutaneous (in Vivo) and Direct (inVitro) Irradiation with Polychromatic Visible and Infrared Light Photomedicine and LaserSurgery: 24(2) 129-139, 2006